column chromatography principle

When a mixture of mobile phase and sample to be separated are introduced from top of the column, the individual components of mixture move with different rates.column chromatographyThose with lower affinity and adsorption to stationary phase move faster and eluted out first while those with greater adsorption affinity move or travel slower and get eluted out last. The solute molecules adsorb to the column in a reversible manner. The rate of the movement of the components is given as follows R= Rate of movement of a component / Rate of movement of mobile phase. i.e. it is the ratio of distance moved by solute to the distance moved by solvent. source:studyread.com/